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. 2010 Aug 4;11:29. doi: 10.1186/1471-2091-11-29

Figure 4.

Figure 4

GCN2-dependent effect upon Trp depletion is linked to aromatic amino acid biosynthesis. (A) Simplified schematic of aromatic amino acid anabolism indicating where excess amino acids inhibit biosynthesis according to reference [11]. The crucial step is the feedback inhibition of the first step (red lines). (B) Growth curve of gcn2Δ with depletion of Trp, Tyr and Phe showing growth recovery in complete media versus Trp depleted media. (C) Heatmap of the wild type and gcn2Δ strains depleted of all three aromatic amino acids. Red arrow indicates tRNATrp and black arrows indicate tRNAPhe and tRNATyr. (D) Histogram of the relative charging level before and 60 min after triple amino acid depletion for the gcn2Δ strain.