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Biochemical properties of recombinant BmCL1 expressed in Pichia pastoris. (A) SDS-PAGE of BmCL1 purified on ion exchange chromatography, as described in Methods. (B) Thermal inactivation at 68°C at pH 4.5. (C) Determination of the cleavage sites of SF 57-67 by BmCL1 using LC/MS.
