Figure 5. Developmental interaction between MHC class I and cognate inhibitory receptors ‘educate’ NK cells to respond to missing self.
Shown are the results of assays in which peripheral blood NK cells are cultured with target cells of the class I deficient 221 B cell line. Using flow cytometry selected subsets (either expressing CD94:NKG2A or KIR3DL1) of NK cells were analysed for presence of intracellular interferon-γ. Blood donors were selected based upon their HLA class I and KIR types. All donors carried the Bw4 epitope. For further details see Yawata et al [52].