Fig. 3.

SDS–PAGE analysis of purified double-labeled protein produced by larger-scale dialysis reaction. Lane M in panels A and B correspond to Precision Plus Protein Marker (Bio-rad). In panel A, lane 1 was loaded 1 μL of purified ¹⁵N-,¹³C-enriched human ubiquitin in NMR buffer (40 μL preparative volume). In panel B lane 1 was loaded 10 μL of Purified HR3597B in elution buffer (1,900 μL preparative volume) and in lane 2, 1 μL of purified HR3597B in NMR buffer (40 μL preparative volume). Lanes 2–5 of panel A contain 0.5, 1.0, 2.0 and 3.0 μg of lysozyme and Lanes 3–5 of panel B contain 0.3, 1.0 and 2.0 μg of lysozyme