Table 1.
Expression and solubility screening results for E. coli intracellular and Promega TNT WGCF expression
E. coli | Total constructs | WGCF ES ≥ 8 | % salvage (%) | |
---|---|---|---|---|
Exp. | Sol. | |||
− | − | 34 | 19 | 46 |
+ | − | 9 | 6 | 67 |
+ | + | 16 | 15 | 94 |
59 | 40 | 68 |
The scoring protocol is explained in the text