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. 2010 Jun 24;11(3):201–209. doi: 10.1007/s10969-010-9093-8

Table 2.

Labeling efficiency of ubiqutin produced using the modified Promega TNT WGCF system with two different concentrations of labeled amino acids in the reaction mixture

Peptide sequence Position 0.1 mM labeled aa’s (%) 1 mM labeled aa’s (%)
EGIPPDQQR 44–52 68.9 93.7
ESTLHLVLR 74–82 73.4 96.4
IQDKEGIPPDQQR 40–52 66.6 94.0
TITLEVEPSDTIENVK 22–37 77.2 93.2