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. Author manuscript; available in PMC: 2010 Aug 14.
Published in final edited form as: Biomaterials. 2009 Jan 21;30(13):2507–2515. doi: 10.1016/j.biomaterials.2009.01.007

Fig. 1.

Fig. 1

EB structure and microsphere incorporation. (A) Day 10 EBs exhibited a smooth exterior in SEM micrographs, while bisected EBs displayed a dense outer layer (B, C). (D–F), Microspheres labeled with CellTracker Red were observed in multiple focal planes (30 μm between images), while soluble treatment of CellTracker Green labeled the outermost 1–2 cell layers. (G–I) EBs formed at 40 rpm with 1:2 (G), 2:1 (H) and 5:1 (I) ratios of CellTracker Red microspheres-to-cells displayed increasing levels of microsphere incorporation. (J) Quantification of microsphere incorporation in populations of lysed EBs demonstrated that lower rotary speed and higher microsphere to cell ratios increased microsphere incorporation (n = 3, mean ± standard deviation).