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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Lab Invest. 2010 May 10;90(9):1325–1338. doi: 10.1038/labinvest.2010.99

Figure 4.

Figure 4

Figure 4

Figure 4

Immunohistological analysis of tumors. Immunohistochemistry on tumors from vehicle-, resveratrol, 5-FU, and resveratrol+5-FU-treated mice were performed using specific antibodies against CK-19 (A), PCNA (B), and TUNEL (C). Representative photomicrographs of the immunoreactivity are shown (magnification X40) and positive staining is indicated by arrows. Semi-quantitative analysis of PCNA immunoreactivity was performed and data was expressed as average (± SEM) PCNA positive nuclei per field (B) and the asterisk denotes significance (p<0.05) compared to vehicle-treated tumors and the # denotes significance (p<0.05) from 5-FU treated tumors. PCNA expression in the tumors was also assessed by real time PCR (B). Data are expressed as average ± SEM (n=3). Asterisk denotes significance (p<0.05) compared to vehicle-treated tumors. Semi-quantitative analysis of TUNEL positive nuclei was assessed and expressed as average (± SEM) PCNA positive nuclei per field (C) and the asterisk denotes significance (p<0.05) compared to vehicle-treated tumors and the # denotes significance (p<0.05) from 5-FU treated tumors.