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. Author manuscript; available in PMC: 2011 Mar 1.

Published in final edited form as: Lab Invest. 2010 May 10;90(9):1325–1338. doi: 10.1038/labinvest.2010.99

Specific knockdown of Cyp1b1 expression renders cholangiocarcinoma cells more susceptible to chemotherapeutic agents. Mz-ChA-1 cells were stably-transfected with Cyp1b1 shRNA vectors. The expression of Cyp1b1 was assessed in the mock-transfected cell line (Mz-Neo Neg) and the cell line containing the Cyp1b1 shRNA (Mz-Cyp1b1 shRNA) real time PCR and immunoblotting (A). Data are expressed as average ± SEM after correction for GAPDH expression or β-Actin respectively. * denotes significance (p<0.05) from Mz-ChA-1 cells. The effect of chemotherapy treatment on cell proliferation was assessed by MTS assays (B). The three cell lines (Mz-ChA-1, Mz-Neo Neg and Mz-Cyp1b1 shRNA) were treated with 5-FU (10 μM to 30 μM), Gemcitabine (10 μM to 30 μM), or Mitomycin C (1 μM to 10 μM) for 48 hr and data are expressed as fold change in proliferation (average ± SEM, n=7). * denotes p<0.05 compared to basal treatment within each cell line and # denotes significance compared to the effects of these chemotherapeutic agents compared to Mz-ChA-1 cells.

Specific knockdown of Cyp1b1 expression renders cholangiocarcinoma cells more susceptible to chemotherapeutic agents. Mz-ChA-1 cells were stably-transfected with Cyp1b1 shRNA vectors. The expression of Cyp1b1 was assessed in the mock-transfected cell line (Mz-Neo Neg) and the cell line containing the Cyp1b1 shRNA (Mz-Cyp1b1 shRNA) real time PCR and immunoblotting (A). Data are expressed as average ± SEM after correction for GAPDH expression or β-Actin respectively. * denotes significance (p<0.05) from Mz-ChA-1 cells. The effect of chemotherapy treatment on cell proliferation was assessed by MTS assays (B). The three cell lines (Mz-ChA-1, Mz-Neo Neg and Mz-Cyp1b1 shRNA) were treated with 5-FU (10 μM to 30 μM), Gemcitabine (10 μM to 30 μM), or Mitomycin C (1 μM to 10 μM) for 48 hr and data are expressed as fold change in proliferation (average ± SEM, n=7). * denotes p<0.05 compared to basal treatment within each cell line and # denotes significance compared to the effects of these chemotherapeutic agents compared to Mz-ChA-1 cells.