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. 2009 Dec;23(12):1948–1962. doi: 10.1210/me.2009-0095

Table 1.

Primer sequences of genes analyzed in RT-PCR and qRT-PCR

Name Accession no. Amplicon size (bp) Sense primer Antisense primer
Primers for RT-PCR
    αENaC AF112185 555 CTAATGATGCTGGACCACACC AAAGCGTCTGTTCCGTGATGC
    βENaC AF112186 631 GCCAGTGAAGAAGTACCTGC CCTGGGTGGCACTGGTGAA
    γENaC AF112187 670 AAGAATCTGCCGGTTCGAGGC TACCACTCCTGGATGGCATTG
Primers for q RT-PCR
    18S X00686 66 CCCTGCCCTTTGTACACACC CGATCCGAGGGCCTCACTA
    mMR M36074 153 ATGGAAACCACACGGTGACCT AGCCTCATCTCCACACACCAAG
    αENaC AF112185 150 GGACTGGAAAATCGGCTTCC TAGAGCAGGCGAGGTGTCG
    Sgk1 AF205855 150 TCACTTCTCATTCCAGACCGC ATAGCCCAAGGCACTGGCTA
    TonEBP AY050663 127 CCTCTCCTCACCGTCGTCATC CTCCCACCTCCTTCTCATTCTG
    AldoR BC085310 79 GGCCGTGAAAGTTGCTATTGA GGTCCCGGGCTGTGAGAT
    Tis11b NM_007564.3 100 CGACACACCAGATCCTAGTCCTT TGCATAAAACTTCGCTCAAGTCA

The abbreviations of the genes, their GenBank or National Center for Biotechnology Information accession number, and 5′- to 3′-nucleotide sequences of the sense and antisense primers are presented.