Table 1.
Name | Accession no. | Amplicon size (bp) | Sense primer | Antisense primer | |||
---|---|---|---|---|---|---|---|
Primers for RT-PCR | |||||||
αENaC | AF112185 | 555 | CTAATGATGCTGGACCACACC | AAAGCGTCTGTTCCGTGATGC | |||
βENaC | AF112186 | 631 | GCCAGTGAAGAAGTACCTGC | CCTGGGTGGCACTGGTGAA | |||
γENaC | AF112187 | 670 | AAGAATCTGCCGGTTCGAGGC | TACCACTCCTGGATGGCATTG | |||
Primers for q RT-PCR | |||||||
18S | X00686 | 66 | CCCTGCCCTTTGTACACACC | CGATCCGAGGGCCTCACTA | |||
mMR | M36074 | 153 | ATGGAAACCACACGGTGACCT | AGCCTCATCTCCACACACCAAG | |||
αENaC | AF112185 | 150 | GGACTGGAAAATCGGCTTCC | TAGAGCAGGCGAGGTGTCG | |||
Sgk1 | AF205855 | 150 | TCACTTCTCATTCCAGACCGC | ATAGCCCAAGGCACTGGCTA | |||
TonEBP | AY050663 | 127 | CCTCTCCTCACCGTCGTCATC | CTCCCACCTCCTTCTCATTCTG | |||
AldoR | BC085310 | 79 | GGCCGTGAAAGTTGCTATTGA | GGTCCCGGGCTGTGAGAT | |||
Tis11b | NM_007564.3 | 100 | CGACACACCAGATCCTAGTCCTT | TGCATAAAACTTCGCTCAAGTCA |
The abbreviations of the genes, their GenBank or National Center for Biotechnology Information accession number, and 5′- to 3′-nucleotide sequences of the sense and antisense primers are presented.