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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Mol Cancer Ther. 2010 Jul 27;9(8):2354–2364. doi: 10.1158/1535-7163.MCT-10-0207

Figure 5. Multiplex Optical Imaging of a Hairless Rhabdomyosarcoma Model.

Figure 5

A, From left to right: photograph, luciferase luminescence (scale 2.8×105 to 8.0×108 p/sec/cm2/sr), IR-820 fluorescence (scale 3.2×105 to 1.6×109 p/sec/cm2/sr) and MMPsense fluorescence (scale 0 to 2.8×109 p/sec/cm2/sr) for a mouse bearing a rhabdomyosarcoma of the left foot. Viable tumor is shown with arrowheads. A metastatic lesion is shown by an asterisk (*). MMPsense activity is shown by an arrow at the advancing front of the tumor. Apparent MMPsense signal in the abdomen is artifact attributable to alfalfa in mouse chow. B, repeat imaging for a more restricted field of view. Scales: luminescence (2.8×104 to 8.6×108 p/sec/cm2/sr), IR-820 fluorescence (2.1×107 to 3.4×109 p/sec/cm2/sr) and MMPsense fluorescence (0 to 4.9×109 p/sec/cm2/sr). C, necropsy photomicrograph demonstrating an enlarged subcutaneous lymph node that overlied the spleen. D, H&E of primary tumor consistent with rhabdomyosarcoma (400×). E to F, Immunohistochemistry positive for nuclear myogenin and cytoplasmic desmin, respectively (400×). G, H&E of lymph node shown in A, B and C (20×). H, Close-up of G (200×). I, Myogenin positive rhabdomyosarcoma cells in the lymph node (400×).