. Author manuscript; available in PMC: 2011 Mar 15.

Published in final edited form as: Anal Chem. 2010 Mar 15;82(6):2520–2528. doi: 10.1021/ac100010h

Table 2.

Properties of Separated Peptides and their Retardation Factors R_f in the First and Second Dimension Separations

peptide	mol. mass	pI ^a)	H.i.^b)	R_f in 1^st D	R_f in 2_nd D
Val-Tyr-Val	379.5	5.9	71.7	0.87	0.44
leucine enkephalin	555.6	5.9	52.0	0.41	0.34
angiotensin II	1046.2	6.8	28.5	0.66	0.56
bradykinin	1060.2	12.0	2.6	0.59	0.60

^a)

^b)

Hydrophobicity index at pH 7.0 from http://www.sigma-genosys.com.