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. Author manuscript; available in PMC: 2011 Aug 15.
Published in final edited form as: Dev Biol. 2010 Jun 8;344(2):827–835. doi: 10.1016/j.ydbio.2010.05.516

Fig. 5. Physical interaction of EGL-5 and POP-1.

Fig. 5

(A, B) Yeast two-hybrid interaction of EGL-5 and POP-1. (A) BD-POP-1 and AD-EGL-5 transformed yeast cells can grow on -Leu/-Trp/-His media like the positive control BD-53 and AD-T and unlike either negative control, BD-POP-1 and AD-T or BD-53 and AD-EGL-5. (B) Quantitative Beta-Galactosidase assay demonstrating BD-POP-1 and AD-EGL-5 have 3-fold higher Beta-Galactosidase activity than the negative controls. Error bars indicate standard deviation. (C, D) GST-pulldown of EGL-5 by POP-1 and POP-1 by EGL-5. (C) GST-EGL-5 can pull down POP-1 protein but not Intersex. GST-POP-1 can also pull down POP-1. GST-DMRT6 and GST alone pull down POP-1 is much weaker than either GST-EGL-5 or GST-POP-1. (D) GST-POP-1 can also pull down EGL-5 and not Intersex more that GST-DMRT6 or GST alone, however, not as strongly as GST-EGL-5.