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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Biomed Pharmacother. 2010 Feb 24;64(7):493–498. doi: 10.1016/j.biopha.2010.01.003

Figure 4. Effect of NOV-002 on plasma redox potential and free sulfhydryls.

Figure 4

Mice were treated with 250 mg/kg NOV-002 (i.p.). Orbital blood was collected at 0, 5, 10, 15, 30 and 240 min. A) The Eh GSSG/GSH was calculated from the GSSG and GSH concentrations using the Nernst equation. B) Schematic illustrating how the fluorescent probe, ThioGlo™, binds to free thiols. S-Glutathionylated cysteine residues cannot bind ThioGlo and thereby have lower fluorescence intensities. C) Free protein thiol content of plasma proteins was measured at 0, 5, 10, 15 and 30 min following 250 mg/kg (i.p.). D) Free protein thiol content was measured in control (black) and NOV-002 treated (white) tissues 30 minutes following treatment. The ThioGlo-1 emission (at 513nm) for each treatment group was averaged and plotted as mean ± SD (n = 6); (*=p≤0.001).