Table 6.

Immunohistochemical staining of SCAs

SCA case no.	LH/FSH	ACTH	ACTH/LH colocalized	α-GSU	Tpit (%)	NeuroD1 (%)	SF-1 (%)	ACTH/SF-1 colocalized	DAX-1 (%)
1	−	+	n/a	+	−	+++	+++	+	+++
2	−	+	n/a	−	−	+++	+++	n/a	+++
3	−	+	n/a	−	−	+++	+++	−	+++
4	+	+	−	+	+	+++	+++	+	+++
5	−	+	n/a	+	−	+++	++	n/a	+++
6	−	+	n/a	+	+	+++	+++ (nuc)	+	+++
7	−	+	n/a	+	+	+++	+++	+	+++
8	+	+	−	+	−	+++	+++	+	+++
9	+	+	+	−	+	+++	+++ (nuc)	+	+++
10	−	+	n/a	+	+	+++	++	+	+++
10 (1st recurrence)	−	+	n/a	n/a	−	n/a	n/a	n/a	++
10 (2nd recurrence)	−	+	n/a	−	−	+++	+++	+	+++
11	+	+	n/a	n/a	−	+++	+++ (nuc)	+	+++
11 (1st recurrence)	+	+	+	+	−	+++	+++ (nuc)	+	+++
12	−	+	n/a	+	+	+++	+++	+	+++
13	+	+	n/a	+	−	+++	++ (nuc)	n/a	+++
14	−	+	n/a	−	−	+++	+++	−	+++
15	−	+	n/a	+	+	+++	+	n/a	+++ (cyto)
16	+	+	n/a	+	−	+++	++	+	+++
17	−	+	n/a	+	+	+++	++	+	+++
18	+	+	−	−	−	+++	+++	+	+++

Shown is scoring for each immunostained transcription factor

Staining for LH, FSH, ACTH, and α-GSU was not quantified and are scored as either present (+) or absent (−)

Staining for Tpit, NeuroD1, and DAX-1 is nuclear unless otherwise noted. Staining for SF-1 is both nuclear and cytoplasmic unless otherwise noted. For quantification of Tpit, NeuroD1, SF-1, and DAX-1, + = <25%; ++ = 25–50%; +++ = 50–100% percent positive staining according to incidence of positively stained cells

% percentage of cells, Nuc nuclear, Cyto cytoplasmic, n/a not available or not applicable