Fig. 1.

The variation in $s_{\mathit{\text{complex}}}^{*}$ with iron loading of HoSF and HuHF. Curve a: Calculated line for HoSF using equation (9) a described in Materials and Methods. The linear-regression is within the thickness of the line giving s_complex = 0.00882 · n_Fe +15.4 (R = 0.9999). Curve b: Data for sucrose gradient fractionated HoSF ferritin from MP Biomedicals. The linear regression through the 11 data points gives s_complex = (0.0115 ±0.0003) · n_Fe + 15.4 (R=0.982). Curve c: Data for monomeric HuHF reconstituted from apoHuHF with iron in 50 Fe²⁺ additions. The linear regression through the 5 data points gives s_complex (monomer) = (0.0115 ± 0.0003) · n_Fe + 16.6 (R = 0.995). Curve d: Data for dimeric HuHF reconstituted from apoHuHF with 50 Fe²⁺ additions as for curve c. The linear regression through the 5 data points gives s_complex (dimer) = (0.0181 ± 0.0005) · n_Fe + 23.9 (R = 0.996).