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. Author manuscript; available in PMC: 2010 Aug 16.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2009 Apr 1;50(8):3802–3807. doi: 10.1167/iovs.08-2417

Figure 1. Analysis of T cell surface activation markers expression and its proliferative capacity.

Figure 1

Draining LN were harvested from DE vs. normal (NL) mice; single-cell suspensions were prepared and dual stained with anti-CD4 FITC (A) or anti-CD8 FITC (B) and either one of the activation marker antibodies anti-CD69 PE, or anti-CD154 PE. Data shown are representative of five independent experiments. (C) CD4+ T cells were sorted from the draining LN of DE vs. NL mice using the MACS cell isolation kit. CD4+ T effector cells were cocultured with T cell-depleted syngeneic splenocytes and anti-CD3 antibody for three days. Proliferative response was assessed by BrdU incorporation assay. Results are presented as optical density(OD) reading ± SEM. Data shown are representative of three independent experiments.