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. Author manuscript; available in PMC: 2010 Aug 16.
Published in final edited form as: Methods Enzymol. 2009;455:157–192. doi: 10.1016/S0076-6879(08)04206-7

Figure 6.4. Time course of ATP hydrolysis and ADP-Pi burst of myosin V.

Figure 6.4

Time course of ADP-Pi formation by a single-headed myosin V construct after mixing with 100 µM ATP. The solid line is the best fit of the data to Eq. (6.9) with an observed rate constant of 84 ± 13 s−1 and burst amplitude of 0.43 ± 0.03 Pi/myosin. Data are from (De La Cruz et al., 2000b).