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. Author manuscript; available in PMC: 2011 Aug 6.
Published in final edited form as: Cell. 2010 Aug 6;142(3):444–455. doi: 10.1016/j.cell.2010.06.039

Figure 4.

Figure 4

CENP-E phosphorylation by Aurora is required for congression of polar chromosomes. (A) Drug treatment scheme to enrich mono-oriented chromosomes near spindle poles. (B) Diagram illustrating attachment status of chromosomes following treatment with drug regime in (A). (C, D) Immunofluorescence images of drug-treated cells in which endogenous CENP-E was replaced with either WT or T422A MycLAP-CENP-E. KNL-1 (Green); DNA (Red). (E) Percentage of cells from (C,D) with polar chromosomes. (F) Number of polar chromosomes in cells from (C,D) displaying chromosome misalignment. Bars represent the mean of four independent experiments. ***, P < 0.0001 by t test. Error bars represent SEM. See also Figure S7 and Movie S4.