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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Nat Chem Biol. 2010 Apr 25;6(6):418–423. doi: 10.1038/nchembio.351

Table 1.

Comparison of Wzy activity among repeating unit donors with variable lipid moieties. The numbers refer to the radioactivity of O-polysaccharide-PP-lipid against the total radioactivity in the paper chromatography assay [Number/100 = (total radioactivity of the chromatography paper – radioactivity of paper squares where input RU-PP-lipid was located)/total radioactivity of the chromatography paper]. The reactions were allowed to proceed for 4 hours with three replicates being performed for each lipid analog. Percentages shown are the means ± standard error of two independent experiments with triplicate determinations.

Lipid Geometry Structure Percent (%)
Undecaprenol 7 cis, 3 trans graphic file with name nihms176585t1.jpg 51 ± 5
Heptaprenol 4 cis, 2 trans graphic file with name nihms176585t2.jpg 34 ± 4
Pentaprenol 2 cis, 2 trans graphic file with name nihms176585t3.jpg 15 ± 2
Cis-Pentaprenol 4 cis, 0 trans graphic file with name nihms176585t4.jpg 29 ± 4
Solanesol 0 cis, 8 trans graphic file with name nihms176585t5.jpg 0
MS-Pentaprenol 1 cis, 2 trans graphic file with name nihms176585t6.jpg 3 ± 1