Table 3.

Apparent dissociation constants K_d (µM) for the binding of KA-AMC to various RNA constructs^a.

SL1-fl ED	SL1-s ED	SL1-yf KD	SL1-yb KD	SL2	SL3
22.6	47.6	22.9	26.3	47.5	65.1
(2.5)	(2.1)	(1.1)	(1.9)	(2.4)	(0.4)

^aApparent dissociations constants were determined based on fluorescence quenching of small molecules upon RNA titration as explained in Materials and Methods. Standard deviations are shown in parentheses, determined in 2–4 independent experiments.