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The role of integrins in PBEF and IL-8 responses to stretching was shown by addition of a blocking peptide (RGD) to WISH cells during static and cyclic stretch/release (n=5). (A) PBEF (C) IL-8 gene expression with static stretch and (B) PBEF and (D) IL-8 with 2 h of cyclic stretch/release. An example experiment completed with primary isolated AEC from one patient showing gene expression for (E) PBEF and (F) IL-8. Values are mean +/-SEM. P<0.05 for dissimilar letters.
