Table 1.
Effect of macromolecular crowding on folding energetics of P85A CRABP Ia
20° C | 37° C | |||||
---|---|---|---|---|---|---|
Ficoll 70 (g·l−1) | m-value (cal·mol-1·M−1) | Cm (M) | ΔG° (cal·mol−1) | m-value (cal·mol-1·M−1) | Cm (M) | ΔG° (cal·mol−1) |
0 | 1568 ± 109 | 5.36 ± 0.02 | 8401 ± 580 | 1698 ± 127 | 4.27 ± 0.02 | 7252 ± 537 |
75 | 1448 ± 143 | 5.61 ± 0.03 | 8123 ± 791 | 1551 ± 92 | 4.48 ± 0.02 | 6951 ± 412 |
150 | 1326 ± 118 | 5.99 ± 0.04 | 7942 ± 681 | 1477 ± 90 | 4.81 ± 0.02 | 7100 ± 431 |
The parameter values plus the standard errors at each condition are the result of a global fitting of independent measurements (three to four data sets in general, and six for 37 °C/150 g/l Ficoll).