(A) Real time PCR for various ECM components in sham hearts (S) and the border zone (BZ) or remote (R) region of the infarct reveals attenuated expression of collagen genes in the BZ of MRTF-A−/− hearts.
(B) The cytokines TGFβ-1, -2, and -3 do not display significant alterations in expression in the BZ between WT and MRTF-A−/− hearts.
(C) The stress responsive ANF gene displays reduced expression in MRTF-A−/− hearts. TnC levels are elevated to similar levels in the BZ of both WT and MRTF-A−/− animals. n=4 WT and 4 MRTF-A−/− hearts for infarct groups and 2 shams. Error bars represent the SEM.