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. Author manuscript; available in PMC: 2011 Jul 23.
Published in final edited form as: Circ Res. 2010 Jun 17;107(2):294–304. doi: 10.1161/CIRCRESAHA.110.223172

Figure 5. MRTF-A regulates the expression of smooth muscle markers in CFs.

Figure 5

(A) Quantitative Real Time PCR reveals myocardin is exclusively expressed in CMCs, while MRTF-A is present in both CMCs and CFs at similar levels.

(B) Quantitative Real Time RT-PCR reveals SMA and SM22 are significantly enriched in CFs over-expressing MRTF-A relative to β-gal infected control CFs. Error bars represent the SEM.

(C) Indirect immunofluorescence of CFs demonstrates MRTF-A over-expression results in the enrichment of SMA into organized stress fibers as compared with Ad-β-gal infected CFs, which display primarily cortical actin SMA staining. Scale bar = 25 μm.