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. Author manuscript; available in PMC: 2011 Jul 23.
Published in final edited form as: Circ Res. 2010 Jun 17;107(2):294–304. doi: 10.1161/CIRCRESAHA.110.223172

Figure 6. Regulation of MRTF-A nuclear localization and activity in CFs by TGFβ-1 and the ROCK inhibitor, Y-27632.

Figure 6

(A) Immunocytochemistry for SMA in CFs grown in serum free (SF) media or media supplemented with TGFβ-1 (10ng/ml), Y-27632 (10μM) or both TGFβ-1 and Y-27632. All images were captured using identical exposure settings. Scale bar = 25 μm.

(B) Immunocytochemical detection of Flag-tagged MRTF-A in CFs after 24 hours of growth in SF media or media supplemented with TGFβ-1, Y-27632 or both TGFβ-1 and Y-27632. Scale bar = 100 μm.

(C) Quantification of the subcellular localization of Flag-MRTF-A under various culture conditions. Subcellular localization of Flag-MRTF-A was scored for approximately 20 random fields of view for each condition. Error bars represent the SEM (* denotes p-value < 0.01; † denotes p-value < 0.05).