Tilorone induces the expression of canonical HIF-activated genes in HT22 cells (A) or primary neurons (B). (A) Total RNA was collected following treatment DFO (100 µM) or Tilorone (10 µM). Real-time polymerase chain reaction (RT-PCR) using specific primers determined the level of mRNA. (B) Primary neurons were exposed to normoxia (control), canonical HIF activators (1% hypoxia, CoCl2, or DFO), or tilorone or its analogs. Total RNA was collected. Quantitative PCR using specific primers for VEGF, Epo, and LDH (known HIF targets) was performed. Results shown are mean ± SEM; P < 0.05.