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. Author manuscript; available in PMC: 2010 Aug 16.
Published in final edited form as: Ann N Y Acad Sci. 2008 Dec;1147:383–394. doi: 10.1196/annals.1427.033

Figure 4.

Figure 4

Tilorone stabilizes HIF-1 protein levels in brains of animals in a dose-dependent manner (A) and increases hypoxia response element (HRE)–luciferase expression in rat brains (B). (A) Immunoblot analysis with an antibody to HIF-1α in lysates from brains of nontreated (control) animals or following treatment with indicated doses of tilorone or 1% oxygen. (B) Bioluminescence imaging of animals treated with tilorone analog R-10,874-DA (100 mg/kg body weight) was injected intraperitoneally 24 h prior to IVIS imaging (n = 3). Control animals (n = 3) received i.p. saline injection at the same time. (C) Quantification of emitted light (see Materials and Methods for details). Results shown are mean ± SEM.