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. Author manuscript; available in PMC: 2010 Aug 16.
Published in final edited form as: Circ Cardiovasc Genet. 2009 Nov 17;3(1):78–87. doi: 10.1161/CIRCGENETICS.109.871236

Figure 3.

Figure 3

Characterization of ATP-β posttranslational modifications. A, Distribution of changing ATP-β on the 2DE gels. The unmodified form (marked with a thicker arrow) and fragments are indicated by arrows and accompanied by fold change values. B, Representative dephosphorylation gel for ATP-β. DHF is shown in red (Cy5), CRT in green (Cy3), and the AP-treated pool (AP) in blue (Cy2). C, Representative BN-PAGE/Western blot for ATP-β. Biological replicates are shown (3 sham-operated controls, 5 DHF, and 4 CRT). D, Typical MS/MS profile for the phosphopeptide FT*QAGSEVSALLGR. The MS/MS spectrum is provided, together with a schematic in which the mass values for all b- and y-ions series are reported.

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