Skip to main content
. Author manuscript; available in PMC: 2010 Aug 16.
Published in final edited form as: Circ Cardiovasc Genet. 2009 Nov 17;3(1):78–87. doi: 10.1161/CIRCGENETICS.109.871236

Figure 4.

Figure 4

Mitochondrial bioenergetic parameters. Mitochondrial oxygen consumption was measured in the presence of glutamate/ malate (A) and succinate (B) as substrates. Respiration was monitored in basal state, or state 4 (S4); state 3 (S3), induced by the addition of 300 μM ADP; and uncoupled state, induced by the addition of protonophore 2,4-dinitrophenol (10 μM, uncoupled state). C, Respiratory control ratios. D, ADP/oxygen ratios (*P<0.05, **P<0.001 vs CRT, ‡P<0.05, and ‡‡P<0.005 vs shams). E, Representative image of the in-gel ATPase assay (n=3). Specific activity is reported as the density of white PbHPO4 bands (product) normalized for the amount of complex V as indicated by coomassie blue G250 stain (*P=0.02 vs shams and P=0.05 vs DHF).

HHS Vulnerability Disclosure