FIG. 7.
Glucose infusion increases cyclin D2 abundance and nuclear localization. A: Islets from mice infused with saline or glucose for 4 days show that cyclin D2 protein abundance significantly increases; cyclin D1, cyclin D3, and cdk4 are unchanged. B: Immunohistochemistry for cyclin D2 reveals pronounced nuclear localization in islets from glucose-infused mice. C: Real-time PCR demonstrates no difference in mRNA abundance of D-type cyclins and cdks 4 and 6 in islets from saline- or glucose-infused mice. Scale bars = 20 μm (10 μm in insets). Data are means ± SE.