Figure 5.
Aberrant ipsilateral calyces express presynaptic markers. A, In this mutant ipsilateral MNTB (P9 EphB2−/−;EphB3−/−), RDA labeling reveals an aberrant calyx (arrow). B, Expression of synapsin I (SynI) in the same section as in A. C, Merge of A and B indicates that the RDA-labeled aberrant calyx is also positive for synapsin I (yellow). D, In a different mutant ipsilateral MNTB (P26 ephrin-B2lacZ/+), RDA labeling reveals axons (red) and two aberrant calyces (arrows). E, SV2 immunofluorescence in the same section as in D. F, Merge of D and E indicates that the RDA-labeled calyces (arrows) are also positive for SV2 (yellow). Scale bar: (in F) A–F, 20 μm.