Figure 2. Salivary protection of oral tissue and neutralization of protection.
(A) A significant decrease in C. albicans CFU counts recovered from tongues infected in saliva (Saliva) compared to control tongues (Control) with PBS. However, no significant effect on CFU counts was seen when saliva was pre-treated with purified proteases (Saliva+Saps). (B) Similarly, the anti-candidal effect of saliva was compromised following its pre-incubation with C. albicans cells (Saliva+CA), suggesting proteolysis and deactivation of a protective salivary component by treatment of saliva with Saps and C. albicans cells. Results represent the averages of data from 4 subjects tested on several occasions. Error bars indicate the standard errors of the means. No significant difference is seen between control and treated saliva (p>0.05). For all other values p<0.05.