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. Author manuscript; available in PMC: 2010 Aug 16.
Published in final edited form as: J Control Release. 2008 Apr 23;129(2):117–123. doi: 10.1016/j.jconrel.2008.04.012

Fig. 1.

Fig. 1

(a) An overview of the cloning strategy used to make a pET21b expression vector containing the gene encoding cKH-FGF2. The FGF2 gene was cloned into pET21b vector in between EcoRI and Hindlll restriction sites to make pET21b-FGF2 vector. The synthesized cKH gene was cloned into pET21b-FGF2 vector in between Ndel and EcoRI restriction sites to make pET21b-cKH-FGF2 expression vector, (b) The corresponding amino acid sequence of the cloned cKH-FGF2 gene. The cKH sequence is shown in bold and the amino acid sequence of FGF2 is underlined.