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(A) Stopped-flow UV-visible spectroscopic monitoring (900 scans, 85 sec) of the double-mixing reaction between preformed DHP B Compound ES (10 μM; 500 ms) and a 7-fold molar excess of DCQ at pH 7. (B) Calculated UV-visible spectra for Compound ES (black) and a ferric/oxyferrous DHP B mixture (grey) are shown; the rapid-scanning data from panel A were compiled and fitted to a one-step, two species sequential irreversible model using the Specfit global analysis program.
