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Spectroelectrochemical determination of the formal reduction potential of the ferric/ferrous couple in DHP B. (A) The UV-visible spectroelectrochemical plot of DHP B at various applied potentials (Eapplied versus SHE) are shown. In the absence of dioxygen, ferric DHP B (λmax = 407 nm) converted to the ferrous enzyme (λmax = 432 nm) as the applied reduction potential was lowered from 497 mV to −130 mV. (B) The corresponding Nernst plot of the data in panel A.
