. Author manuscript; available in PMC: 2011 Aug 10.

Published in final edited form as: Biochemistry. 2010 Aug 10;49(31):6600–6616. doi: 10.1021/bi100407v

Table 3.

Kinetics data for the oxidative catalytic reaction of the different co-substrates: TCP, TBP and TFP with 0.5 μM DHP B and 150 μM of the corresponding co-substrates and varying H₂O₂ concentrations in 100 mM KPB pH 7

Co-Substrate	DHP B				DHP A			Ratio ^c k_catB/k_catA
Co-Substrate	K_M^H₂O₂ (μM)	K_M^TXP (μM)	k_cat (s⁻¹)	k_cat/K_M^H₂O₂ (μM⁻¹ s⁻¹)	K_M^H₂O₂ (μM)	k_cat (s⁻¹)	k_cat/K_M^H₂O₂ (μM⁻¹ s⁻¹)	Ratio ^c k_catB/k_catA

TFP ^a	96 ± 18	N/A	N/A	N/A	59 ± 10	N/A	N/A	2.1
TCP ^a	22 ± 2	210 ± 23	1.53 ± 0.03	0.070	23 ± 1	0.61 ± 0.01	0.027	2.6
TCP ^b	35 ± 6	N/A	1.17 ± 0.05	0.034	16 ± 1	0.57 ± 0.01	0.036	2.1
TBP ^a	63 ± 15	315 ± 11	1.3 ± 0.1	0.021	11 ± 1	0.30 ± 0.01	0.027	4.3

N/A = not available

ferric starting oxidation state

oxyferrous starting oxidation state

as ratio of initial rate