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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Arthritis Rheum. 2010 Aug;62(8):2424–2431. doi: 10.1002/art.27534

Figure 3.

Figure 3

P60 PLAD protein inhibited lupus serum-induced monocyte differentiation into dendritic cells (DCs). (A). Representative pictures of monocytes from C57BL/6 mice cultured with serum from lupus MRL/lpr mice, normal C57BL/6 mice for 40 hours. (B) Expression of CD40 and CD80 on DCs measured by flow cytometry. (C) Representative pictures of monocytes from C57BL/6 mice cultured with serum from lupus MRL/lpr mice with or without IgG depletion for 40 hours. (D) Immunofluorescent staining of DCs in C57BL/6 mice skin with FITC conjugated anti-CD11c Ab 3d after intradermal injection of serum (100 µl) from lupus MRL/lpr or C57BL/6 mice. (E) Cumulative data of DCs differentiated from monocytes of C57BL/6 mouse treated with lupus MRl/lpr serum for 40 hours in the presence of PBS, P60 PLAD (100 µg) or P80 PLAD (100 µg). (F) Cumulative data of DCs differentiated from monocytes of mice with TNFR1 or TNFR2 gene deficiency and wild type mice were cultured with serum from lupus MRL/lpr mice for 40 hours.