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. Author manuscript; available in PMC: 2010 Aug 16.
Published in final edited form as: Cell Motil Cytoskeleton. 2009 Aug;66(8):469–482. doi: 10.1002/cm.20369

Fig. 2.

Fig. 2

Co-localization studies of FAP73-HA, FAP134-HA, and PACRG-HA.

(A) Methanol-fixed cell expressing FAP73-HA stained with anti-HA (a) and anti-IFT46 (b). The merged image is shown in c. (B) Isolated flagella with protruding central pair (arrowheads in b) from a strain lacking endogenous FAP134 and expressing FAP134-HA, stained with anti-HA (a) and anti-tubulin (b). The merged image is shown in c. (C) Same as in B but from strain 4.3 expressing PACRG-HA. (D) Split axonemes of strain 4.3 expressing PACRG-HA, stained with anti-HA (a, d, g) and anti-tubulin (b, e, h). The merged images (c, f, i) show that staining representing PACRG-HA is strongly reduced or absent from some outer doublet microtubules. Strain 4.3 used for the images in C and D carries a PACRG RNAi construct in addition to the PACRG-HA vector. See Supplementary Fig. 1 for a more detailed characterization of this strain. Bars = 5 µm.