Table I.
Genes selected for HA-tagging
| gene (v2.0) |
protein ID | protein name |
size of PCR product (bp) |
upstream of start codon (bp) |
start codon - pen- ultimate codon (bp) |
human homologue |
BLAST E value |
|---|---|---|---|---|---|---|---|
| C_20334 | EDP07596 | PACRG | 3120 | 1483 | 1637 | NP_689623 | 8e-66 |
| C_30068 | EDP04330 | FAP146 | 4267 | 1628 | 2639 | NP_115633 | 3e-16 |
| C_310134 | EDO99184 | FAP173 | 4384 | 883 | 3501 | NP_038470* | 3e-11 |
| C_320063 | EDP08000 | FAP232 | 2508 | 1522 | 986 | NP_057210 | 3e-19 |
| C_380055 | EDP03601 | FAP134 | 5155 | 1666 | 3489 | NP_112584 | 1e-56 |
| C_410061 | EDP09736 | FAP122 | 3795 | 1469 | 2326 | BAB15699 | 8e-16 |
| C_450085 | EDP05720 | FAP207 | 2802 | 819 | 1983 | AAH57760 | 3e-30 |
| C_630058 | EDP05376 | FAP52 | 5062 | 1567 | 3495 | AAH25392 | 1e-161 |
| C_650031 | EDO98890 | FAP73 | 3842 | 1720 | 2122 | NP_653282 | 4e-17 |
| C_60149 | EDO99770 | FAP295 | 4254 | 340 | 3914 | NP_006250 | 5e-90 |
The gene numbers in version 2.0 of the C. reinhardtii genome (JGI), the protein names, details of the PCR products, putative human homologues, and the E values obtained in BLAST searches between the human and C. reinhardtii gene products are shown. The size of the PCR products does not include the overhangs and restriction sites added to the primers for cloning of the PCR product.
*
Although NP_038470 is the closest match to C_310134 in humans, C_310134 is much more closely related to band 7 proteins in Leishmania and plants.