Table II.
Proteins tagged by HA in this study
| protein | size without 3xHA (aa) |
predicted mass incl. 3xHA/ apparent mass in SDS-PAGE (Da) |
localization | transformants (positive/tested) |
flagellar proteome unique peptides/fraction |
predicted domains /motifs |
|---|---|---|---|---|---|---|
| PACRG | 308 | 38913/44000 | fla/c | 21/57 | 24/axoneme | PACRG superfamily |
| FAP146 | 381 | 45719/50000 | fla/bb | 9/24 | 9/KCl extract | - |
| FAP173 | 303 | 37435/42000 | fla/bb/c | 5/24 | 7/membrane + matrix and KCl extract | Band 7 superfamily |
| FAP232 | 190 | 25156/35000 | fla/bb | 26/48 | 5/membrane + matrix | Galactose-binding domain-like |
| FAP134 | 525 | 65205/70000 | fla/c | 3/40 | 10/axoneme | Leucine-rich repeats |
| FAP122 | 374 | 47414/54000 | fla/c | 7/36 | 11/axoneme and tergitol insoluble | - |
| FAP207 | 257 | 33174/39000 | fla | 20/24 | 6/axoneme and tergitol insoluble | MORN ? |
| FAP52 | 634 | 73271/79000 | fla/c | 4/12 | 30/axoneme | WD40 repeats |
| FAP73 | 309 | 40410/49000 | fla/bb | 4/38 | 18/KCl extract | coiled-coil |
| FAP295 | 719 | 85471/90000 | fla/c | 4/48 | 31/KCl extract | cGMP-dependent protein kinase |
The protein names, sizes, predicted molecular mass of the HA-tagged products, molecular mass observed on western blots, localization in this study, number of tested and positive transformants, number of unique peptides and predominate fraction in which they were found in the proteomic analysis of flagella, and predicted domains of the gene products are shown. aa, amino acids; fla, flagella; c, cell body; bb, near the basal bodies.