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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: J Immunol. 2010 May 5;184(11):6103–6113. doi: 10.4049/jimmunol.0804356

Figure 4. Expression of α4 and β1 integrins in male, female and castrated male MBP-primed T cells and spleens.

Figure 4

MBP-primed T cells of female, male and castrated male mice were analyzed for the expression of α4, β1 and β7 integrins by semiquantitative RT-PCR (A). The mRNA expression of α4 and β1 in female, male and castrated male MBP-primed T cells were further analyzed by quantitative real-time PCR (B). Data are mean ± S.D. of three different experiments. Female, male and castrated male MBP-primed T cells were treated with appropriately diluted FITC-labeled antibodies against α4 or β1 or β1 and CD3 for 30 min followed by FACS analysis (C & D). Splenic cross sections of MBP-immunized female and male mice were double immunolabeled with antibodies against CD3 and β1 (E). Setting of the microscope was strictly unaltered during the whole study. Figures are representative of three independent experiments. (F) Cells positive for CD3, β1 or CD3 & β1 were counted in five splenic sections (3 images per slide) of each of three mice per group.