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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: J Immunol. 2010 May 5;184(11):6103–6113. doi: 10.4049/jimmunol.0804356

Figure 8. Effect of estrogen (ET), progesterone (PT), testosterone (TT), and dihydrotestosterone (DHT) on the surface expression of β1 integrin in female MBP-primed T cells.

Figure 8

Female MBP-primed T cells treated with ET, PT, TT, and DHT for 72 h during MBP priming were incubated with appropriately diluted FITC-labeled anti-VLA-4 β1 and PE-labeled annexin-V for 1 h followed by two-color FACS analysis. Figures represent three independent experiments (A). Mean fluorescence intensity of β1 in FITC-positive cells were calculated by using CellQuest software (B). Data are ±S.D. of three independent experiments.