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. Author manuscript; available in PMC: 2010 Aug 16.
Published in final edited form as: Eur J Neurosci. 2001 Oct;14(7):1082–1090. doi: 10.1046/j.0953-816x.2001.01730.x

FIGURE 1.

FIGURE 1

Degenerate oligonucleotides that amplify strychnine-sensitive, glycine receptor α1-α3 cDNAs. Sequences in public databases were compared using standard procedures (see Methods) to define highly conserved regions between the various α isoforms. Sequences for degenerate oligos were: upper primer, 5' TTG TTC TTT GCC/T AAT GAG AAG/A GG; and, lower primer, 5’ GCC ATC CAG/A ATG TCA ATA/T GC. Numbers indicate the relative nucleotide positions within the α1(accession # D00833; Grenningloh et al., 1987), α2 (accession # X61159, Kuhse et al., 1991), and α3 cDNAs (accession # M55250, Kuhse et al., 1990). “y” = pyrimidine nucleotide; “r” = purine nucleotide; “w” = A or T nucleotide.