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Schematic of the measurement and reference cells inside the adiabatic housing of the ITC. The measurement and reference cells have an active volume of about 1.4 ml. The instrument maintains a constant temperature difference (dT) between the measurement and reference cells. Aliquots of a ligand solution are added to the measurement cell using a syringe controlled by a motorized injection system. Binding of the ligand to the macromolecule releases or consumes heat.
