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. 2010 May 18;21(6):739–749. doi: 10.1089/hum.2009.190

FIG. 4.

FIG. 4.

GLA can be fused to other targeting ScFvs and used to target Ad5. (A) αEGFR ScFv fused to GLA in cell supernatant was used to target Ad-Red to EGFR-overexpressing cells lines SKOV-3 and MDA-MD-468. Supernatant from 293 cells not secreting the targeting protein was incubated with Ad-Red and used as a negative control. dsRed expression was analyzed after 24 hr by FACS. (B) Purified GLA-αEGFR protein was incubated with Ad-GL-RC or Ad-GL-HB-RC at the indicated GLA-to-hexon molar ratios for 1 hr at 4°C before infection of EGFR-expressing SKOV-3 cells. After 24 hr, in vitro luciferase expression was measured.