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. 2010 May 18;21(6):739–749. doi: 10.1089/hum.2009.190

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Copyright 2010, Mary Ann Liebert, Inc.

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FIG. 6. — (A) In vitro luciferase expression from SKOV-3 cells infected with Ad-GL. SKOV-3 cells have low CAR expression and are not readily infected by Ad at low MOI. A549 cells infected with either Ad-GLA-αEGFR-RD or Ad-LacZ-RD control virus provided secreted protein via a permeable Transwell. Protein secreted by Ad-GLA-αEGFR-RD-infected A549 cells enhanced the infection of SKOV-3 cells by Ad-GL-RC. n = 4. (B) In vitro cell-killing assay for Ad-GLA-αEGFR-RC virus. SKOV-3 cells plated on a 96-well plate were infected with serially diluted Ad-GLA-αEGFR-RC or control Ad-GL-RC virus. Two weeks after infection, cell viability was assessed by MTT assay (n = 4).