Figure 1.

Immunohistochemistry and Congo Red analysis of stomachs from TTR mice treated with doxycycline. Doxycycline was administered in the drinking water (8 mg/Kg/day) for 15 days followed by a suspension period of 15 days (water alone) after which animals were sacrificed, (n = 15); control mice were given water alone (n = 11). The top 2 panels represent CR analyses (arrow pointing at CR birefringence in a non-treated mouse) and MMP-9 IHC. The bottom 2 panels are representative of SAP (arrow pointing at staining which co-localizes with amyloid in a non-treated mouse) and TTR IHC. Scale bar (CR and SAP = 100 μm; MMP-9 and TTR = 200 μm).