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. Author manuscript; available in PMC: 2011 Jul 30.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Jul 1;398(3):525–531. doi: 10.1016/j.bbrc.2010.06.112

Figure 3. HSP90β binding to LAP affects latent TGFβ mediated signaling and cell proliferation.

Figure 3

MG63 cells were transfected with (CAGA)12-luciferase reporter plasmid and the pRL-TK control vector. A, cells were treated with recombinant latent TGF-β1 and different concentrations of HSP90β for 24 h before lysis. B, cells were pre-treated with anti-HSP90β antibody or IgG control and then incubated with latent TGF-β1 for 24 h before lysis. C, MG63 cells were treated with 200 ng/ml latent TGF-β1 with various concentrations of recombinant HSP90β. Before and after 48 h of treatment, cell numbers were counted. D, MG63 cells were starved for 24 h and treated with 200 ng/ml latent TGF-β1 with various concentrations of recombinant HSP90β. Total RNA was extracted from the cells at 24 h after treatment and analyzed by quantitative real-time PCR. Each value represents the mean of triplicate determinations; bars, each value represents the mean of triplicate determinations; bars, mean + SD. n=3, *P < 0.05, **P < 0.01, ***P < 0.001.