Fig. 6.

miR-10a negatively regulates MAP3K7 and βTRC through their 3′ UTR binding site. (A and B) Evolutionarily conserved putative miR-10a binding site within the 3′ UTR of MAP3K7 and βTRC. (C and D) Increased expression of MAP3K7 in miR-10a knockdown HAEC. Gene (n = 7–10) and protein (n = 3) expressions were normalized to GAPDH and β-actin, respectively. (E) Reduced luciferase activity in HEK293 cells overexpressing miR-10a following insertion of miR-10a binding elements cloned from MAP3K7 and βTRC 3′ UTRs. Data represent mean ± SEM. ns, not significant. *P < 0.05.